Before we talk about SAM, BAM and CRAM, we must discuss the software, SAMtools, from which these formats originate.
SAMtools is a suite of utilities that allow for efficient post-processing of short DNA sequence read alignments. The program includes several command line programs such as
index that allow for next-generation sequence data processing.
The SAM, BAM and CRAM file formats come from the use of SAMtools.
The name SAM comes from Sequence Alignment/MAP. In addition to regular sequence reads, SAM includes alignment data that link short reads to a reference sequence. This makes SAM files the choice of format when visualizing short read sequences in genome browsers such as IGV (Integrated Genome Viewer).
The SAM format is simple to parse, generate and check for errors. However, its large file size (~10 gb on average) gets in the way of efficiency. Thus, researchers found a way to compress it into a binary format without losing the ability to manipulate it. BAM contains indexable representation of nucleotide sequence alignments, allowing for intensive data processing in production pipelines.
CRAM is a restructured version of its binary version, with column-orientation.
For more reading on SAM and BAM, head over to the Center for Statistical Genetics
Command Line Kung Fu is packed with dozens of tips and practical real-world examples. You won't find theoretical examples in this book. The examples demonstrate how to solve actual problems. The tactics are easy to find, too. Each chapter covers a specific topic and groups related tips and examples together.$ Check price
Get a comprehensive, in-depth introduction to the core Python language with this hands-on book. Based on author Mark Lutz's popular training course, this updated fifth edition will help you quickly write efficient, high-quality code with Python. It's an ideal way to begin, whether you're new to programming or a professional developer versed in other languages.$ Check price