Bridge PCR is a PCR technique that embeds DNA on a surface for cloning. It is used by Illumina's HiSeq platform.
The DNA is fragmented (through sonication or any other method) and adapters are ligated to both ends.
The DNA is then denatured into single-stranded molecules. These fragments are then floated onto a flow cell which have corresponding adapter sequences that permit binding.
When the DNA strands are placed onto the slide, they attach to their corresponding adapter sequences.
Add dNTPs, and DNA polymerase enzyme to elongate DNA strands.
Denaturation the newly formed DNA strands and repeat until dense clusters of dsDNA are generated in each channel of flow cell.
The reverse strands are then cleaved and washed away.
After Bridge PCR is conducted on the flow cell, Illumina uses fluorescently labeled dNTP's to detect each nucleotide bases.
So for example, if a red fluorescent light goes off, then we know it's an A. If a blue light goes off, then we know it's a G, and so forth (colors here aren't accurate but you get the picture).
However, the signal produced by the synthesis of one dNTP on a strands is not enough to be detected. This is why we need to amplify the DNA sequences and producing a dense amount of sequences per area on the flow cell.
We'll see how Illumina is able to sequence millions of these dense colonies in parallel.
If you're looking for a fun and easy entry point into bioinformatics algorithms, this book it just for you! Filled with graphics, and written in a light-hearted and humorous story-telling persona, Bioinformatics Algorithms guides you through the intricacies of the problems faced in biology, and the clever solutions used to solve them.$ Check price
This book approaches system administration in a practical way and is an invaluable reference for both new administrators and experienced professionals. It details best practices for every facet of system administration, including storage management, network design and administration, email, web hosting, scripting, and much more.$ Check price